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Increased Ratio of Dietary Carbohydrate to Protein Shifts the Focus of Metabolic Signaling from Skeletal Muscle to Adipose

Increased Ratio of Dietary Carbohydrate to Protein Shifts the Focus of Metabolic Signaling from Skeletal Muscle to Adipose

Suzanne Devkota and Donald K LaymanNutrition & Metabolism 2011, 8:13 doi:10.1186/1743-7075-8-13Published: 4 March 2011

Coolstance copra

Published: 4 March 2011

Background 

The Dietary Reference Intakes (DRI) established acceptable macronutrient distribution ranges (AMDR) for carbohydrates and protein, however little is known about differences in glycemic regulations and metabolic signaling across this range. This study examined metabolic outcomes associated with intake of two diets differing in carbohydrate:protein ratios representing the upper and lower ends of the AMDR. 

Methods 

Adult, male rats were fed either a high carbohydrate (CHO) diet (60% of energy from carbohydrates, 12% protein, 28% fat; n = 30) or a high protein (PRO) diet (35% carbohydrate, 35% protein, 30% fat; n = 30). Rats were meal-fed 3x/d the respective diets for 10 d and then terminated after overnight food deprivation or 30, 60, 90, 120 min post-prandial (PP). Plasma was collected at each of these points to provide a time course for glucose, insulin and C-peptide. Skeletal muscle and adipose tissues were collected at 0, 30 and 90 min for measurements of basal, early and delayed activation of Akt, p70S6K and Erk 1/2. Data were analyzed by two-way ANOVA. 

Results 

The CHO group produced a consistently elevated response in plasma glucose, insulin and C-peptide following the meal through the 120 min time course. In addition, Akt and Erk 1/2 activation in adipose was much higher than in skeletal muscle. Conversely, the PRO group PP glucose response was minimal and insulin maintained a response similar to a biphasic pattern. Tissue responses for the PRO group were greater for Akt and p70S6K signaling in skeletal muscle compared with adipose. 

Conclusion 

Taken together these data suggest that altering CHO:PRO ratios within the AMDR produce different glycemic response patterns accompanied by differential metabolic signaling in skeletal muscle and adipose.

Animals chronically consuming the CHO diet produced greater metabolic signaling in adipose tissue to handle excess glucose and blunted signaling in skeletal muscle consistent with interpretation of insulin resistance. 
Conversely, animals consuming the PRO diet produced greater metabolic signaling in skeletal muscle with little signaling in adipose. 
These data suggest that consuming an increased CHO:PRO diet may have detrimental effects on insulin sensitivity.


Practical application


Coolstance copra has low NSC (11%), ie has low CHO:PRO and so does not significantly increase circulating insulin and glucose levels.

Full paper http://www.nutritionandmetabolism.com/content/pdf/1743-7075-8-13.pdf

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